Description
MellitusVioletC™istheconvenientcontrolcompoundforMellitusVioletprobes1-3™.Withthesamechromophorefluorescentbackbone,butdoesnotchelatedissolvedglucose,MellitusVioletC™servesasanidealcontrolcompoundforall3oftheMellitusViolet™probes.MellitusVioletC™doesnotshowanyresponsetoglucose,fructoseorotherdiols.Similartotheparentprobemolecules,MellitusVioletC™canreADIlybeexcitedat320nm,withanemissioncentredat≈430nmintheblueregionofthevisIBLespectrum.
MellitusVioletC™issolddryandissimplyreconstitutedbyaddingwater/MeOH.Onevialofourprobewilltypicallyallowfor10control-likemeasurementstobeundertaken,whenfurtherdilutedinto3cm3volumeswithinacuvette. 50µLofwater/MeOHand/orbufferistypicallyaddedtoeachdryvial.Subsequently,5µLaliquotsdilutedto3cm3,typicallygiveanoptimumODof≈0.25.
Foroptimumperformance,probevialsshouldbestoredat-2to-8Cwhennotbeingused.Probesolutionsshouldbeusedafterdilution.