Description
MellitusBlue3™showsafluorescenceintensityreductionasafunctionofglucoseconcentrationupto100mMglucose,withaglucosepKaof6.90and5.45for100mMFructose.MellitusBlue3™canreADIlybeexcitedfrom300-400nmwithanemission≈460nm.
MellitusBlue3™issolddryandissimplyreconstitutedbyaddingwater/MeOH.Onevialofourglucoseprobewilltypicallyallowfor10glucosemeasurementstobeundertaken,whenfurtherdilutedinto3cm3volumeswithinacuvette.50µLofwater/MeOHand/orbufferistypicallyaddedtoeachdryvial.Subsequently,5µLaliquotsdilutedto3cm3,typicallygiveanoptimumODof≈0.25.
Foroptimumperformance,probevialsshouldbestoredat-2to-8Cwhennotbeingused.Probesolutionsshouldbeusedafterdilution.WerecommendtheuseofMellitusBlueC™,thecontrolcompoundforMellitusBlue3™,totestforpossIBLebackgroundinterferences,suchasfromfructose,othersugarsanddiolinterference.