Description
MellitusViolet3™showsafluorescenceintensityreductionasafunctionofglucoseconcentrationupto100mMglucose,withaglucosepKaof6.95and5.45for100mMFructose.MellitusViolet3™canreADIlybeexcitedat320nm,withanemission≈430nm.
MellitusViolet3™issolddryandissimplyreconstitutedbyaddingwater/MeOH.Onevialofourglucoseprobewilltypicallyallowfor10glucosemeasurementstobeundertaken,whenfurtherdilutedinto3cm3volumeswithinacuvette.50µLofwater/MeOHand/orbufferistypicallyaddedtoeachdryvial.Subsequently,5µLaliquotsdilutedto3cm3,typicallygiveanoptimumODof≈0.25.
Foroptimumperformance,probevialsshouldbestoredat-2to-8Cwhennotbeingused.Probesolutionsshouldbeusedafterdilution.WerecommendtheuseofMellitusVioletC™,thecontrolcompoundforMellitusViolet3™,totestforpossIBLebackgroundinterferences,suchasfromfructose,othersugarsanddiolinterference.