Description
MellitusViolet2™showsafluorescenceintensityreductionasafunctionofglucoseconcentrationupto100mMglucose,withaglucosepKaof6.85and5.05for100mMFructose.MellitusViolet2™canreADIlybeexcitedat320nm,withanemission≈430nm.
MellitusViolet2™issolddryandissimplyreconstitutedbyaddingwater/MeOH.Onevialofourglucoseprobewilltypicallyallowfor10glucosemeasurementstobeundertaken,whenfurtherdilutedinto3cm3volumeswithinacuvette.50µLofwater/MeOHand/orbufferistypicallyaddedtoeachdryvial.Subsequently,5µLaliquotsdilutedto3cm3,typicallygiveanoptimumODof≈0.25.
Foroptimumperformance,probevialsshouldbestoredat-2to-8Cwhennotbeingused.Probesolutionsshouldbeusedafterdilution.WerecommendtheuseofMellitusVioletC™,thecontrolcompoundforMellitusViolet2™,totestforpossIBLebackgroundinterferences,suchasfromfructose,othersugarsanddiolinterference.