Description
PhosYellow™isahighlyphosphorescentorganicprobewhichcanbeexcitedfrom300-380nm.Inairequilibratedglycerol,thefluorescenceemissionoccursat≈400nmandthephosphorescenceiscentered≈540nm,withafwhmof≈100nm.Thephosphorescencelifetimeinairequilibratedglycerolat22C(em=525nm)is≈120µS.PhosYellow™isreADIlysolubleinmethanol.ethanolandacetone.
PhosYellow™issolddryandissimplyreconstitutedbyaddingMeOHorEtOHorothersuitablesolvent.Onevialofthisprobewilltypicallyallowfor10measurementstobeundertaken: 50µLofMeOHistypicallyaddedtoeachdryvial.Subsequently,ten5µLaliquotsdilutedto3cm3(cuvette),willtypicallygiveanopticaldensity>0.05at320nm(inspectroscopicMeOH).
Foroptimumperformance,probevialsshouldbestoredat-2to-8Cwhennotbeingused.Probesolutionsshouldbeusedafterdilution.
AnUrsaBioScienceshortvideodescribesourreconstitutionprocedure.