Description
PhosYellow2™canreADIlybeexcitedfrom300-380nm.PhosYellow2™showsamuchstrongerphosphorescenceat≈540nmthanPhosYellow™inairequilibratedglycerol,andsimilartoPhosYellow™hasaFWHMof≈100nm.ThePhosphorescencetailsintotheyellowspectralregion,whichaccountsforitsuniqueyellowphosphorescence.Thelifetimeinairequilibratedglycerolis≈113µS.
PhosYellow2™issolddryandissimplyreconstitutedbyaddingMeOH,EtOHoranothersuitablesolvent.Onevialofthisprobewilltypicallyallowfor10measurementstobeundertaken: 50µLofMeOHistypicallyaddedtoeachdryvial.Subsequently,ten5µLaliquotsdilutedto3cm3(cuvette),willtypicallygiveanopticaldensity>0.1at320nm(inspectroscopicMeOH).
Foroptimumperformance,probevialsshouldbestoredat-2to-8Cwhennotbeingused.Probesolutionsshouldbeusedafterdilution.
AnUrsaBioScienceshortvideodescribesourreconstitutionprocedure.