Description
MethuselahGreenCarboxy™hassimilarfluorescenceandphosphorescencepropertiesasMethuselahGreen2™,whereboththefluorescenceandphosphorescencebandsofMethuselahGreen2™canbeexcitedat290-350nm,withthePhosphorescenceemissioncenteredaround530nm. Thefluorescencebandiscenteredataround410nm.Whenexcitedat280nm,thelifetimeoftheprobeis1.67msascomparedto350nmexcitation,≈1.069ms.
MethuselahGreenCarboxy™issolddryandissimplyreconstitutedbyadding50µLofeitherwater/MeOH/EtOHand/orbuffer.Onevialofoursensitizerwilltypicallyallowfor10measurementstobeundertaken,when5µLfromthe50µLisfurtherdilutedinto3cm3volumeswithinacuvette,theODat320nmtypically>0.1.
Foroptimumperformance,probevialsshouldbestoredat-2to-8°Cwhennotbeingused. Probesolutionsshouldbeusedafterdilution.
AnUrsaBioScienceshortvideodescribesourreconstitutionprocedure.