Description
SensitizerBlue™isawatersolublefluorescentandphosphorescentprobewithanabsorbancefrom260-350nm,withλmax≈320nm. ThebluefluorescencebandofSensitizerBlue™from380-460nm,canreADIlybeexcitedintherange300-350nm.ThePhosphorescencespectraiscenteredaround510nmwhichcanreadilyexcitedfrom280-320nm. Thephosphorescencelifetimeincreaseswhentheexcitationwavelengthisreducedfrom320to280nm,2.81and3.07msrespectivelyinair-equilibratedglycerolat0C.
SensitizerBlue™issolddryandissimplyreconstitutedbyadding50µLofeitherwater/MeOH/EtOHand/orbuffer. Onevialofoursensitizerwilltypicallyallowfor10measurementstobeundertaken,when5µLfromthe50µLisfurtherdilutedinto3cm3volumeswithinacuvette.
Foroptimumperformance,probevialsshouldbestoredat-2to-8Cwhennotbeingused. Probesolutionsshouldbeusedafterdilution.
AnUrsaBioScienceshortvideodescribesourreconstitutionprocedure.