Description
ViscousBlue420™isawatersolubleprobewhichcanreADIlybeexcitedintherange250-400nm,withλmaxAbs≈330nminMethanolandablueemissionbandcenteredat≈420nm(glycerol).At20Cthelifetimeisbi-exponential(inglycerol),0.899ns(56%),and1.70ns(44%),withameanlifetimeof1.13ns.At50Cthelifetimesaremuchshorter,0.492ns(82.7%)and1.16ns(17.3%),withameanlifetimeof0.547ns.
ViscousBlue420™hasaviscositydependentemissioncenteredat420nm,theintensityofwhichisdependentonviscosity,overabroadrangeofviscosity.Assolutionviscosityincreases(solutiontemperaturedecreases),thefluorescenceincreases.
ViscousBlue420™issolddryandissimplyreconstitutedbyaddingwater/MeOH/EtOHand/orbuffer.OnevialofourViscosityProbewilltypicallyallowfor10measurementstobeundertaken;50µLofwaterorMeOHand/orbufferistypicallyaddedtoeachdryvial.Subsequently,ten5µLaliquotsdilutedto3cm3,typicallygiveanopticaldensity>0.1at330nm(inMeOH).
Foroptimumperformance,probevialsshouldbestoredat-2to-8Cwhennotbeingused.Probesolutionsshouldbeusedafterdilution.
AnUrsaBioScienceshortvideodescribesourreconstitutionprocedure.